C. Metabolic Processes · Metabolic ProcessesMetabolism Beyond the Cell: Ecosystems, the Carbon Cycle and Technologies
C1.1 C1.2 C3.4 A1.9 A2.1About 70 minutes
Learning goals- I can analyse how photosynthesis and respiration connect living things with the atmosphere, oceans and soils.
- I can explain how changes to metabolic processes (eutrophication, burning fossil fuels) affect abiotic systems.
- I can describe technologies based on metabolic processes, such as fermentation, anaerobic digestion, wastewater treatment and biofuels.
- I can evaluate the benefits and drawbacks of a metabolic technology for society and the environment.
Words to know
biotic
Living parts of an ecosystem, such as plants, animals, fungi and bacteria.
abiotic
Non-living parts of an ecosystem, such as air, water, soil, light and temperature.
carbon cycle
The movement of carbon among the atmosphere, living things, oceans, soils, rocks and fossil fuels.
eutrophication
Enrichment of a body of water with nutrients (especially phosphorus and nitrogen), causing excessive growth of algae and, later, oxygen depletion.
hypoxia
A condition of very low dissolved oxygen in water.
anaerobic digestion
The breakdown of organic matter by microbes in the absence of oxygen, producing biogas (mainly methane and CO₂) and digestate.
biogas
A mixture of mainly methane (CH₄) and carbon dioxide produced by anaerobic digestion; it can be burned as fuel.
biofuel
A fuel made from recently living organisms, such as ethanol from corn fermentation or biogas from manure.
activated sludge
A wastewater treatment process in which air is bubbled through sewage so aerobic microbes respire the organic matter.
Your cells and the whole planet
The air you breathe is about 21% oxygen because of billions of years of photosynthesis. The CO₂ that is warming the climate comes mostly from burning coal, oil and natural gas, which are the remains of ancient photosynthesizers. Even a summer "dead zone" in Lake Erie comes down to photosynthesis and respiration getting out of balance. Expectation C1.1 asks you to analyse how metabolic processes work in, and connect, biotic (living) and abiotic (non-living) systems. This final lesson zooms out from the cell to the planet, and then looks at technologies that put metabolism to work.
Remember from Lessons 8 and 15
Photosynthesis takes in CO₂ and releases O₂; aerobic respiration does the reverse. Fermentation and anaerobic respiration happen without O₂ and are carried out by many microbes (Lesson 8). Matter cycles; energy flows (Lesson 15).
Metabolism shaped Earth's atmosphere
Early Earth's atmosphere had almost no free O₂. When cyanobacteria evolved oxygen-producing photosynthesis, O₂ slowly accumulated; geologists date a major rise in atmospheric oxygen (the Great Oxidation Event) to roughly 2.4 billion years ago. That change made aerobic respiration, with its much larger ATP yield, possible, and eventually allowed large, active, multicellular life. It also created the ozone layer (O₃ formed from O₂), which shields land organisms from ultraviolet radiation.
Today, photosynthesis by land plants and ocean phytoplankton removes huge amounts of CO₂ each year, and respiration by all organisms (especially decomposers) returns a similar amount. This balance links the biosphere (biotic) with the atmosphere, hydrosphere and lithosphere (abiotic).
The carbon cycle
- Photosynthesis moves carbon from atmospheric CO₂ into organic molecules.
- Feeding moves it from producers to consumers.
- Cellular respiration (by producers, consumers and decomposers) returns it to the atmosphere as CO₂.
- Decomposers (bacteria and fungi) respire the carbon in dead matter and wastes; in waterlogged, oxygen-poor places (bogs, wetlands, deep sediments) decomposition is slow and anaerobic, so carbon builds up as peat. Canada's northern peatlands store enormous amounts of carbon this way.
- Over millions of years, buried organic matter can become fossil fuels. Combustion releases that ancient carbon rapidly.
- The oceans exchange CO₂ with the air; dissolved CO₂ forms carbonic acid (as in your BTB tests), and rising CO₂ is making seawater slightly more acidic (ocean acidification).
Because burning fossil fuels adds carbon faster than photosynthesis and the oceans can remove it, atmospheric CO₂ has risen from about 280 ppm before the Industrial Revolution to about 430 ppm in 2026, the main cause of current climate change (you studied climate change in Grade 10).
The carbon cycle. Photosynthesis moves carbon from the air into living things; respiration (by producers, consumers and decomposers) and combustion return it. Fossil fuels store carbon from ancient photosynthesis.When the balance tips: eutrophication and dead zones
Phosphorus and nitrogen from farm fertilizer, manure and sewage can wash into lakes. These nutrients act like fertilizer for algae and cyanobacteria, which bloom at the surface (lots of photosynthesis). When the bloom dies, it sinks, and decomposers respire the dead cells, using up dissolved O₂ in the deeper water. The result can be hypoxia (very low O₂), which kills fish and other organisms, creating a "dead zone".
Lake Erie, the shallowest and warmest of the Great Lakes, is a well-known example: it has had large algal blooms in summer and low-oxygen zones in its central basin. In 2016, Canada and the United States set a target to cut the phosphorus entering Lake Erie by 40% (compared with 2008 levels), and Ontario farmers, municipalities and conservation authorities are working on ways to reduce runoff. Some cyanobacteria in blooms can also release toxins, which is why public health units sometimes issue beach and drinking-water advisories.
How eutrophication leads to a dead zone. Photosynthesis at the surface feeds a burst of aerobic respiration by decomposers below, which uses up the dissolved oxygen.Technologies that use metabolism
Fermentation in food and drink. Bread, yogurt, cheese, sauerkraut, kimchi, soy sauce and vinegar all depend on microbial metabolism. Food scientists control temperature, pH and the microbe used to get the product they want.
Fuel ethanol. Yeast fermentation turns sugars from corn (and, increasingly, from other plant material) into ethanol, which is blended into gasoline. Benefits: renewable; the CO₂ released was recently taken from the air by the crop. Drawbacks: growing, fertilizing, harvesting and processing the crop uses energy and land that could grow food, so the net climate benefit depends on how it is produced.
Anaerobic digestion and biogas. In sealed tanks with no O₂, anaerobic bacteria and methane-producing archaea break down manure, food waste and sewage sludge into biogas (mostly methane), which can be burned for heat and electricity. Some Ontario farms and municipalities run digesters. Capturing the methane is important, because methane is a much more powerful greenhouse gas than CO₂ if it escapes.
Wastewater treatment. In the activated sludge process, blowers bubble air through sewage so that aerobic microbes can respire dissolved organic matter into CO₂ and water. This removes the material that would otherwise cause oxygen depletion in rivers and lakes.
Composting uses aerobic decomposers (turning the pile adds O₂). Green-bin programs in many Ontario cities send food waste to composting or anaerobic-digestion facilities.
Research frontiers: scientists are working on "artificial leaves" that split water using sunlight, and on engineering crops for more efficient photosynthesis (for example, research aimed at giving rice C₄-like features, or at reducing photorespiration). These are still in development.
An anaerobic digester. Without oxygen, microbes break down organic wastes into biogas (methane and CO₂) for energy and digestate for fertilizer.Worked exampleLet's analyse a biotic-abiotic interaction
In a small Ontario pond, dissolved O₂ is measured every 3 hours on a sunny July day: 06:00: 5 mg/L; 09:00: 7 mg/L; 12:00: 10 mg/L; 15:00: 12 mg/L; 18:00: 11 mg/L; 21:00: 8 mg/L; 24:00: 6 mg/L; 03:00: 4 mg/L. Explain the pattern using photosynthesis and respiration.
- Daytime rise (06:00 to 15:00): algae and aquatic plants photosynthesize; O₂ production exceeds all the respiration in the pond, so dissolved O₂ rises, peaking in mid-afternoon.
- Evening and night fall (18:00 to 03:00): no light, so no photosynthesis; plants, animals and decomposers keep respiring, using O₂.
- The lowest O₂ is just before dawn (about 4 mg/L), after a whole night of respiration.
- Link to eutrophication: in a nutrient-rich pond with a heavy bloom, night-time respiration can drop O₂ so low that fish suffocate before dawn.
O₂ rises in daylight because photosynthesis > respiration and falls at night because only respiration continues. This is a biotic process (metabolism) changing an abiotic factor (dissolved O₂).
Worked exampleLet's evaluate a technology
A dairy farm in Ontario is deciding whether to install an anaerobic digester for its manure. Evaluate the proposal using at least three criteria.
- Environmental: captures methane that would otherwise escape from stored manure (methane is a strong greenhouse gas); the biogas can replace fossil fuels; digestate is a fertilizer with less odour. Risk: leaks of methane must be prevented.
- Economic: cost of building and maintaining the digester vs income or savings from electricity and heat, and possibly from accepting food waste from nearby businesses.
- Social: less odour for neighbours; local renewable energy; requires trained operators (a career opportunity).
- Judgement: if the farm is large enough to cover the costs and can manage the system safely, the digester is likely a good choice, because it turns a waste and a source of greenhouse gas into energy and fertilizer.
A strong evaluation uses criteria (environmental, economic, social), considers both benefits and drawbacks, and ends with a reasoned judgement.
Just for funCows burp out methane made by microbes living in their stomachs, which ferment the grass the cows can't digest themselves. Researchers are testing feed additives, such as certain seaweeds, to cut these emissions. It may be the only area of science where "reducing burps" is a serious climate strategy.
Hands-on activityCompost in a bottle: aerobic vs anaerobic decomposition
You need: 2 clean 2 L plastic bottles with the tops cut off (safety note: cut slowly with sturdy scissors; the cut plastic edge can be sharp), garden soil, chopped vegetable scraps (no meat or dairy), dry leaves or shredded paper, water, plastic wrap, an elastic band, a kitchen thermometer, gloves.
- Fill both bottles with equal layers: soil, scraps, leaves, repeated, and moisten them equally (damp like a wrung-out sponge).
- Bottle A (aerobic): poke 10 small air holes in the sides with a pencil and stir the mixture every 2 days.
- Bottle B (mostly anaerobic): pack it down tightly, add a little extra water, and seal the top with plastic wrap and an elastic band. Don't stir it.
- Keep both in the same place. Twice a week for 3 weeks, record temperature in the middle, appearance, and smell (waft carefully; wash hands after).
- Display your qualitative observations in a table and your temperatures on a line graph.
Questions:
- Which bottle warmed more? Which process released the heat?
- Which bottle smelled worse? What kinds of microbes and products might cause this?
- How does this relate to dead zones in lakes, and to how cities run green-bin programs?
What to look for: Bottle A usually warms more and breaks down faster with an earthy smell, because aerobic respiration releases more energy (heat). Bottle B decomposes more slowly and often smells sour or rotten, because fermentation and anaerobic respiration produce acids and sulfur compounds. This mirrors oxygen-poor lake bottoms and shows why compost piles are turned (to add O₂). Dispose of both mixtures in a garden composter or green bin.
Your turn
1Give two ways that photosynthesis affects abiotic parts of Earth.
Any two: it adds O₂ to the atmosphere (and water); it removes CO₂ from the air and oceans; it created the O₂ that formed the ozone layer; it stores carbon that can become fossil fuels or peat.
2Explain how decomposers connect biotic and abiotic parts of the carbon cycle.
Decomposers respire the organic carbon in dead organisms and wastes (biotic), releasing it as CO₂ to the air or water (abiotic), and returning nutrients to the soil.
3Describe the sequence of events in eutrophication.
Excess nutrients (P, N) enter water → algal bloom (photosynthesis) → algae die → decomposers respire them, using O₂ → hypoxia → fish and other organisms die.
4Why does burning fossil fuels increase atmospheric CO₂ even though photosynthesis removes CO₂?
Combustion releases carbon that was stored for millions of years, faster than photosynthesis and the oceans can remove it, so CO₂ accumulates in the atmosphere.
5Explain how anaerobic digestion produces energy from manure.
In a sealed tank with no O₂, anaerobic microbes break down organic matter and methane-producing archaea make biogas (CH₄ + CO₂), which is burned for heat and electricity.
6Why is air bubbled through sewage in the activated sludge process?
To supply O₂ so aerobic microbes can respire the organic matter into CO₂ and water, removing it before the water is released, which prevents O₂ depletion in rivers and lakes.
7Give one benefit and one drawback of corn-based fuel ethanol.
Benefit: renewable; the CO₂ released was recently absorbed by the corn. Drawback: growing and processing corn uses energy, fertilizer and land that could grow food, which reduces the net benefit.
More practice: warm-up
1What are the two main gases in biogas?
Methane (CH₄) and carbon dioxide (CO₂).
Very low dissolved oxygen in water.
3Which nutrient is the main target for reducing algal blooms in Lake Erie?
4Is composting mainly aerobic or anaerobic?
Aerobic (that's why piles are turned).
5About what was the atmospheric CO₂ level before the Industrial Revolution?
More practice: on your own
1Explain why fish kills in eutrophic ponds often happen just before dawn on calm summer nights.
At night there's no photosynthesis, but algae, animals and decomposers keep respiring, so O₂ falls all night and is lowest before dawn. Warm water holds less O₂, and calm weather means little O₂ mixes in from the air.
2Peat bogs store large amounts of carbon. Use your knowledge of metabolism to explain why, and predict what happens if a bog is drained.
Waterlogged peat has very little O₂, so decomposition is slow and anaerobic; dead plant matter accumulates. If the bog is drained, O₂ enters, aerobic decomposers respire the peat much faster, and large amounts of CO₂ are released.
3Explain why capturing methane in a digester is better for the climate than letting manure decompose in an open pile or lagoon.
In open storage, some manure decomposes anaerobically and methane escapes to the atmosphere, where it traps far more heat than CO₂ per molecule. A digester captures the methane and burns it, converting it to CO₂ (a weaker greenhouse gas) while producing useful energy that can replace fossil fuels.
4Choose one fermentation-based food and describe the microbe, the process and why it preserves the food.
Example: yogurt. Bacteria such as Lactobacillus carry out lactate fermentation of milk sugar, producing lactic acid. The acid lowers pH, thickening the milk proteins and making it hard for harmful microbes to grow, which preserves the food.
5Explain how an increase in global temperature could change the balance of photosynthesis and respiration in Canada's northern forests and soils.
Warmer temperatures may lengthen the growing season (more photosynthesis), but they also speed up respiration by decomposers in soils and thawing permafrost, which releases stored carbon. If decomposer respiration rises more than photosynthesis, these ecosystems could become a net source of CO₂ rather than a sink.
Challenge
1A lake has 8.0 mg/L dissolved O₂. A bloom of algae dies, adding organic matter whose complete aerobic decomposition would need 12.0 mg/L of O₂. Assuming no O₂ is added, what happens? What factors in a real lake could change the outcome?
Demand (12.0 mg/L) exceeds supply (8.0 mg/L), so decomposers would use all the O₂ (falling to 0 mg/L) with organic matter still left; remaining decomposition would be anaerobic (producing substances such as H₂S and methane), and fish would die or leave. In a real lake, wind mixing, inflow of oxygenated water, photosynthesis by surviving algae near the surface, temperature (cold water holds more O₂), and the depth of the lake would change the outcome.
2Evaluate the claim: "Biofuels are carbon-neutral, so they have no effect on climate."
The claim oversimplifies. Burning a biofuel releases CO₂ that the plants recently absorbed, so the combustion itself is roughly balanced. But growing the crop (fertilizer production, farm machinery), processing it (heating for distillation) and transporting it all release CO₂ and other greenhouse gases (such as N₂O from fertilizer), and land-use changes can release stored carbon. The net effect depends on the feedstock and process; some biofuels reduce emissions substantially, others very little.
3Design a simple investigation to test whether adding phosphorus increases algal growth and lowers night-time dissolved O₂ in pond water. Include variables, controls and a safety or ethical note.
Use several identical clear jars of the same pond water. Independent: amount of phosphate fertilizer added (e.g. 0, low, medium, high), with at least 3 jars each. Dependent: algal growth (water greenness or turbidity) and dissolved O₂ at dawn (using a school O₂ probe or test kit). Controlled: volume, light (same window), temperature, time. Control: jars with no phosphorus. Measure over 1 to 2 weeks. Safety/ethics: wear gloves and wash hands (pond water can contain microbes and cyanobacterial toxins); don't release treated water back into a natural pond.
Check your understandingQuiz
1. Which process removes CO₂ from the atmosphere?
2. In eutrophication, dissolved O₂ in deep water falls mainly because:
3. Biogas from an anaerobic digester is mainly:
4. Why is air pumped through sewage in the activated sludge process?
5. Why do waterlogged peatlands store large amounts of carbon?
6. In a pond on a sunny day, dissolved O₂ is usually highest in:
For parents and teachersBig idea: C1.1 asks students to analyse how metabolic processes function in and connect biotic and abiotic systems (atmosphere, water, soil), and C1.2 to relate this knowledge to the community. This lesson covers the carbon cycle, eutrophication and technologies based on metabolism (A1.9: evaluating claims; A2.1: careers such as environmental scientist, water and wastewater operator, food scientist and bioenergy engineer).
How to help: The compost-bottle activity takes 3 weeks; the cut bottle edges can be sharp, and everyone should wear gloves and wash hands. Talk about local examples: your municipality's green-bin program, a nearby lake or river, or a farm digester. Encourage your teen to use criteria and evidence when evaluating technologies, rather than calling them simply "good" or "bad".
Common difficulties:
- Blaming algae themselves for using up O₂ in dead zones; it is mainly the decomposers' respiration after the bloom dies.
- Thinking biofuels have zero emissions.
- Forgetting that decomposers respire too, and that anaerobic decomposition produces methane.